The literature data base has been updated with one paper:
Venables et al. used a high throughput RT-PCR screen to find genes that are alternatively spliced during fibroblast reprogramming and differentiation. The genes MBNL1 and RBFOX2 were found to control nearly all the alternative splicing events observed during late mesoderm differentiation, including the alternative splicing of FNIP1 which is controlled by MBNL1. In primary fibroblasts, a shorter isoform of FNIP1 – lacking the final 84 bp of exon 6, corresponding to amino acids 208-235 – predominated. Reprogramming of these fibroblasts to induced pluripotent stem cells (iPSCs) caused the longer, canonical, isoform of FNIP1 to predominate. This splicing pattern was fully reversed upon in vitro differentiation of iPSCs into fibroblasts. Sequence analysis across species shows that two thirds of the alternatively spliced exons found in this study (including FNIP1) arose during the emergence of jawed vertebrates, indicating that alternative splicing of these genes may play an important role in the evolution and physiology of jawed vertebrates.
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