van Slegtenhorst et al. (2007) identified the S. pombe FLCN homologue, BHD1, and used homologous recombination to generate a novel deletion strain. BHD1 only has homology with the N-terminal region of the vertebrate protein (see alignment), but these experiments suggest that yeast FLCN is important for amino acid homeostasis, and potentially activates the mTOR homologue Tor2.
Legend: Alignment of S. pombe and human folliculin. S.pombe folliculin only aligns to the N-terminal region of human folliculin.
The S. cerevisiae orthologues of FLCN and FNIP1/2 are Lst7 and Lst4 respectively. Péli-Gulli et al., (2015) found that Lst7 and Lst4 form a complex that mediates amino acid stimulation of TORC1 signalling.